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Stressgen Biotechnologies grp94
Grp94, supplied by Stressgen Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/grp94/anti+grp94/us12600725-1289-28-29
Average 86 stars, based on 1 article reviews
grp94 - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Inhibitors of the IRE-1/XBP-1 pathway and methods of using thereof
Article Snippet: Antibodies to TCL1 (Cell Signaling), IRE-1 (Cell Signaling), XBP-1 (Santa Cruz), Blimp-1 (Santa Cruz), IRF4 (Cell Signaling), Pax5 (Santa Cruz), Syk (Cell Signaling), phospho-Syk (Tyr525/526) (Cell Signaling), AKT (Cell Signaling), phospho-AKT (Ser473) (Invitrogen), ERK1/2 (Cell Signaling), phospho-ERK1/2 (Thr202/Tyr204) (Cell Signaling), GRP94 (Stressgen), calreticulin (Stressgen), calnexin (Stressgen), phospho-eIF2α (Ser51) (Cell Signaling), eIF2α (Cell Signaling), HSP70 (Stressgen), p97 (Fitzgerald), CHOP (Cell Signaling), actin (Sigma), AID (Cell Signaling), phospho-Iga (Tyr182) (Cell Signaling), μ (SouthemBiotech) and κ (SouthemBiotech) were obtained commercially.

Article Title: Agonist-mediated activation of STING induces apoptosis in malignant B cells
Article Snippet: Purification of mouse B cells and Eμ-TCL1 CLL cells Splenocytes were obtained from mice by mashing the spleens through cell strainers followed by RBC lysis (Qiagen).

Article Title: Selective Grp94 inhibitors and uses thereof
Article Snippet: Flow cytometry analysis was performed using MACSQuant analyzer (Miltenyi Biotec).

Article Title: Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins
Article Snippet: To make SNAPflag-CNX, the vector sequence of CER-CNX sans the Cerulean tag was amplified with primers (forward) 5′-ATG GAC AAA GAC TGC GAA AT-3′ and (reverse) 5′-CTT GTC ATC GTC GTC CTT G-3′, and replaced the CER with SNAPflag that was PCR amplified from pME-puro-SNAP-FLAG-CD59 (gift from Dr. Reika Watanabe, La Jolla Institute for Immunology, San Diego, CA; Addgene plasmid #50374) using the following primers with overhangs for the vector: (forward) 5′-GAC TAC AAG GAC GAC GAT GAC AAG GCA GGAGGCAGCCATGA-3′, and (reverse) 5′-GCT TCA TTT CGC AGT CTT TGT CCA TGG TGG CGA CCG GTG GA-3′.

Cell-Signaling:

Article Title: Secretory IgM exacerbates tumor progression by inducing accumulations of MDSCs in mice
Article Snippet: .. Antibodies against TCL1 (Cell Signaling), IRE-1 (Cell Signaling), XBP-1 (Cell Signaling), Syk (Cell Signaling), phospho-Syk (Tyr525/526) (Cell Signaling), AKT (Cell Signaling), phospho-AKT (Ser473) (Invitrogen), ERK1/2 (Cell Signaling), phospho-ERK1/2 (Thr202/Tyr204) (Cell Signaling), GRP94 (Stressgen), p97 (Fitzgerald), actin (Sigma), phospho-Igα (Tyr182) (Cell Signaling), mouse μ (SouthernBiotech), human μ (SouthernBiotech), and phosphotyrosine (4G10; Millipore) were obtained commercially. .. Anti-μ Fab and F(ab′)2 were purchased from SouthernBiotech.



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(A) Schematic depiction of MBV isolation procedure from in vitro dermal fibroblast cultures. (B) Nanoparticle tracking analysis of diluted MBVs (1:10,000) measures particle size distribution and concentration. (C) Transmission electron micrograph of MBVs reveals vesicle-like morphology. (D) Western blot analysis of Alix (~100kDa), Integrin β1 (~100–130 kDa), CD63 (~35–55 kDa), and <t>GRP94</t> (~100 kDa) expression in MBVs and dermal fibroblast cell lysate. (E) Fluorescence and scanning electron microscopy images of fast-relaxing and slow-relaxing alginate hydrogels with and without MBVs. Top: 5kPa hydrogels encapsulating CFSE-labeled MBVs, * denotes region outside of hydrogel. Bottom: 20kPa hydrogels encapsulating unlabeled MBVs.
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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and <t>Grp94</t> proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.
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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and <t>Grp94</t> proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.
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(A) Schematic depiction of MBV isolation procedure from in vitro dermal fibroblast cultures. (B) Nanoparticle tracking analysis of diluted MBVs (1:10,000) measures particle size distribution and concentration. (C) Transmission electron micrograph of MBVs reveals vesicle-like morphology. (D) Western blot analysis of Alix (~100kDa), Integrin β1 (~100–130 kDa), CD63 (~35–55 kDa), and GRP94 (~100 kDa) expression in MBVs and dermal fibroblast cell lysate. (E) Fluorescence and scanning electron microscopy images of fast-relaxing and slow-relaxing alginate hydrogels with and without MBVs. Top: 5kPa hydrogels encapsulating CFSE-labeled MBVs, * denotes region outside of hydrogel. Bottom: 20kPa hydrogels encapsulating unlabeled MBVs.

Journal: Biomaterials science

Article Title: Matrix stiffness and stress relaxation regulate matrix-bound nanovesicle release from alginate hydrogels

doi: 10.1039/d6bm00832a

Figure Lengend Snippet: (A) Schematic depiction of MBV isolation procedure from in vitro dermal fibroblast cultures. (B) Nanoparticle tracking analysis of diluted MBVs (1:10,000) measures particle size distribution and concentration. (C) Transmission electron micrograph of MBVs reveals vesicle-like morphology. (D) Western blot analysis of Alix (~100kDa), Integrin β1 (~100–130 kDa), CD63 (~35–55 kDa), and GRP94 (~100 kDa) expression in MBVs and dermal fibroblast cell lysate. (E) Fluorescence and scanning electron microscopy images of fast-relaxing and slow-relaxing alginate hydrogels with and without MBVs. Top: 5kPa hydrogels encapsulating CFSE-labeled MBVs, * denotes region outside of hydrogel. Bottom: 20kPa hydrogels encapsulating unlabeled MBVs.

Article Snippet: Primary antibodies were diluted in 5% w/v bovine serum albumin in 1X PBS-T (0.2%), including anti-Integrin β1 (1:1,000, Cell Signaling Technology, 34971), anti-Alix (1:10,000, Proteintech, 12422–1-AP), anti-CD63 (1:1,000, Proteintech, 25682–1-AP), and GRP94 (1:5,000, Proteintech, 14700–1-AP).

Techniques: Isolation, Encapsulation, In Vitro, Analysis, Concentration Assay, Transmission Assay, Morphology, Western Blot, Expressing, Fluorescence, Scanning Electron Microscopy, Labeling, Cell Culture

Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and Grp94 proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.

Journal: Medicine

Article Title: Human amniotic epithelial cell-derived exosomes promote conjunctival goblet cells proliferation and mucin secretion

doi: 10.1097/MD.0000000000049647

Figure Lengend Snippet: Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and Grp94 proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.

Article Snippet: The polyvinylidene fluoride membrane was treated with protein-free rapid blocking solution (PS108P, EpiZyme) for 15 minutes, and then incubated with primary antibodies against exosome markers CD63 (1:1000, ABclonal, A19023) and CD81 (1:1000, ABclonal, A4863), as well as the negative marker Grp94 (1:1000, ABclonal, A0989) overnight.

Techniques: Transmission Assay, Electron Microscopy, Western Blot, Expressing, Derivative Assay